Shandong Binzhou Zhiyuan Biotechnology Co.,Ltd
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    Shandong Binzhou Zhiyuan Biotechnology Co.,Ltd

    Tel: +86-543-8128042

    Fax: +86-543-2605568

    Email: info@cydextrin.com

    Add: Boxing Economic Development Zone, Binzhou, Shandong, China

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Methylated-β-cyclodextrin

Different temperatures and different preparation methods, the hydrolysis of insulin have been studied. In acidic solution, the degradation of insulin mainly deamination, generation deaminated product. This study insulin and methyl-β-cyclodextrin (metβCD) whether the interaction can increase the stability of insulin. Hydrolysis of insulin by HPLC conditions. Lineweaver-Burke equation to calculate the insulin / methyl-β-cyclodextrin inclusion complex stability constants. In addition, using 1H NMR and ESI-MS determination of insulin / metβCD inclusion characteristics. Kinetic parameters indicate metβCD improve its stability for insulin effect. The kinetic results are substituted into the Lineweaver-Burke equation and the Arrhenius equation, the calculated stability constants Kst and the activation energy Ea. Analysis of its 1H NMR, adding cyclodextrin, the inclusion of specific amino acids on insulin aromatic group chemical shifts have changed. Similarly, with the ESI-MS measurement, an increase in the mass spectrum m / z peak. Conclusion is metβCD can effectively increase the stability of insulin.

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Address: Boxing Economic Development Zone, Binzhou, Shandong, China
Tel: +86-0543-2616568
 Fax:+86-543-2605568
 E-mail:info@cydextrin.com
Shandong Binzhou Zhiyuan Biotechnology Co.,Ltd
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